Drosophila Activity Monitor (DAM) system能讓廣大的生物科學(xué)家的監(jiān)測果蠅和類似大小的昆蟲在運動和羽化方面的行為規(guī)律，此系統(tǒng)已被應(yīng)用于突變異種、環(huán)境監(jiān)測和對化學(xué)藥品的敏感性，也可用來展示群居生活中相互間的作用。
果蠅被單獨放置在玻璃瓶皿中，通過紅外光束來探測和計算它們的運動行為，在一定的周期內(nèi)，這些累計的行為數(shù)據(jù)將從監(jiān)視器上傳到計算機中存儲和分析.
*運動監(jiān)視器：用32個內(nèi)徑為 5mm,7mm或者10mm的管子，分別監(jiān)測32個果蠅樣本的行為規(guī)律。一個紅外光束穿過把管子分成兩半，當(dāng)果蠅在管子中來回走動，它們將切割紅外光，這些運動數(shù)據(jù)則被記錄下來。
*羽化監(jiān)視器：將成熟的果蠅蟲卵粘貼在一個朔料圓盤底下，用一個內(nèi)徑100mm的玻璃漏斗來收集蟲卵孵化出來的果蠅。一個紅外監(jiān)測環(huán)安裝在漏斗下方的頸口處，用來監(jiān)測計算每一個通過的果蠅，一個搖晃的機械裝置可以定期的搖晃朔料圓盤，使得孵化出來的果蠅能夠完全從圓盤掉落到玻璃漏斗中。
*群聚監(jiān)視器：用一個內(nèi)徑25mm的玻璃或者朔料小瓶子，裝入多個果蠅，根據(jù)瓶子的長度放置幾個紅外監(jiān)測環(huán)，記錄果蠅運動總數(shù)。
*環(huán)境監(jiān)測器：測量培養(yǎng)器皿內(nèi)的光的強度，溫度和相關(guān)的濕度
*電源：提供一個直流電源用于整個系統(tǒng)，通過一個電 話線網(wǎng)絡(luò)一樣的平行接口，這個電源可以提供給多達(dá)120個監(jiān)視器同時使用
*記錄軟件：提供一個存儲檔案從一個或多個監(jiān)視器同時記錄行為數(shù)據(jù)。一個實驗可連續(xù)記錄數(shù)小時、數(shù)天甚至是幾個星期。
The Drosophila Activity Monitoring System enables a biologist to accurately characterize the locomotor and eclosion behavior rhythms in drosophila fruit flies and similarly-sized insects. The system has been used to screen for mutants, measure environmental and chemical sensitivities, and characterize social interaction, all based on the patterns of physical movement which the system observes and quantifies over time.
The individual flies are placed into glass chambers, where their confined motion may be detected and counted by infrared light beams in one or more activity monitoring units. At periodic intervals, these accumulated activity counts are uploaded from the monitors to a host Macintosh or Windows computer for storage and analysis.
The Locomotor Monitor uses 32 tubes of 5, 7, or 10 mm diameter, and measures the activity rhythms of 32 individual flies. An infrared beam bisects each tube, and detects motion as the flies walk back and forth from end to end.
The Eclosion Monitor uses a 100 mm dia. glass funnel to collect emerging flies as they fall from their pupae cases on the underside of a plastic disk. An infrared ring detector at the funnel neck counts each as it passes by, and a mechanical tapper dislodges periodically those which remain adhered to the disk or funnel walls.
The Population Monitor uses a 25 mm dia. glass or plastic vial to contain a multiplicity of flies, and measures their aggregate movement with ring detectors spaced along the vial length.
The Environment Monitor measures the light intensity, temperature, and relative humidity within an incubator chamber, and provides a continuous archival record of these conditions over the duration of an experiment.
The Power Supply Unit supplies DC power to an entire system of up to 120 monitors using a parallel network of residence-type telephone cables. An optional Light Controller allows up to 6 incubator lights to be independently cycled on and off under computer control.
The DAMSystem Collection Software provides archival storage of the collected activity data from a number of monitors simultaneously, and allows for continuous operation over the hours, days, or weeks of an experimental run.
產(chǎn)品介紹
DAM5M果蠅活動監(jiān)視器可同時測量32只果蠅的自發(fā)活動，可用于果蠅食物（food）、氣味（odor）、方向（orientation）、燈光（light）等位置偏愛（position preference）實驗，每個試管直徑為5mm,當(dāng)果蠅來回飛行時，只要中斷試管1/4紅外光束，就將有一個記錄信號，每個試管有4個獨立的紅外光束，不但可以記錄遺漏的數(shù)，而且紅外到紅外的移動杜絕了原地計數(shù)，還可記錄在每個紅外光束的停留時間（dwell time），內(nèi)置安全的試管固定夾，易于移動和操作，全開放設(shè)計，試管燈光均勻滲透，無陰影。
特點
?8?432個試管用于32只果蠅
?8?4 直徑5mm試管用于黑腹果蠅（drosophila Melanogaster）
?8?4 每個試管有4個獨立的紅外光束
?8?4 生成計數(shù)（Counts）、移動（Moves）、停留時間（Dwell）、休息（Rest）和每個果蠅的位置數(shù)據(jù)
?8?4 內(nèi)置管夾用于快速加載和安全處理試管
?8?4 可折疊緊湊的存儲和轉(zhuǎn)運試管
?8?4 操作一致的明暗燈光
?8?4 精密的管道布局
?8?4 電源，連接線和數(shù)據(jù)采集軟件
?8?4 兼容DAM2,LAM25，DEnM和本公司其他監(jiān)控單元
?8?3 產(chǎn)品規(guī)格
?8?4 試管直徑：5mm
?8?4 試管長度：65mm或較大的
?8?4 尺寸： 33.0 x 8.3 x 2.6 cm
?8?4 質(zhì)量：0.26 kg （不含試管）
?8?4 相鄰紅外間距：9.1mm
?8?4 連接： 4 wire, 6 position, RJ-11電話線接頭
?8?4 9 v直流電源輸入和數(shù)據(jù)傳輸
?8?4 數(shù)據(jù)采集軟件：DAMSystem3，操作平臺：Windows PC 或 Apple Macintosh
?8?4 數(shù)據(jù)類型：移動（Moves）、計數(shù)（Counts）、停留時間（Dwell）、位置（Position）,、休息（Rest）
?8?4 數(shù)據(jù)文件格式：42列 DAMSystem文本
?8?4 操作環(huán)境：正常實驗室，不結(jié)露
The Drosophila Activity Monitor measures the activity rhythms of 32 individual flies, each in its own glass tube. In a typical experiment, a food mixture is placed into one end of the empty tube, and sealed on the outside with a wax coating. The fly is then inserted into the tube, followed by a cotton plug or ventilated plastic cap on the other end. The tube is snapped into the plastic clips of the monitor case, and if the tube is horizontal, an active fly will walk back and forth from one end to the other. Each time that it does so, it breaks an infrared light beam which bisects the tube, perpendicular to its axis. This event is detected by the onboard electronics, and added to the count which is maintained for each channel. At periodic intervals, ranging from 1 second to 1 hour, the DAMSystem software in the host computer acquires from the monitor its set of 32 count totals, and saves them. This archival record of the activity of each fly over the duration of the experiment may be plotted or analyzed, and used, for example, to measure the period and phase of the internal circadian clock of each of the 32 flies. Such measurements have been instrumental to the scientific study of fly behavior, and its genetic basis - the circadian clock being the cornerstone. (See: THE TICK-TOCK OF THE BIOLOGICAL CLOCK. Michael W. Young in Scientific American, Vol. 282 No. 3, March 2000.)
Specifications
? Dimensions: 13.50 x 6.82 x 1.03” (343 x 173 x 26 mm)
? Mass: 1.2 kg
? Glass Tubes: Corning Pyrex standard wall tubing, according to model: DAM 5 = 5 mm diameter DAM10 = 10 mm diameter
? Maximum Tube Length: 3.00” (76 mm)
? Interconnect: 4 wire, 6 position, RJ-11 modular telephone line jack to DAMSystem network for DC power input and data transmission
? Case material: PVC/ABS plastic
Features
?32 independent activity channels per monitor.
?Up to 120 monitors per system provide over 3000 simultaneous monitoring channels.
?Models available for various tube diameters: DAM 5 5 mm tube (for D. melanogaster) DAM10 10 mm tube
?Built-in clips hold tubes in place during handling.
?Recessed tube mounts allow monitors to be safely stacked with tubes installed.
?Telephone-type wiring jack allows easy connection to DAMSystem wiring network.
?Consistent operation in ambient light levels from bright laboratory to total darkness.
?Field-proven design over years of continuous use.
?Optional accessories: Pyrex Glass Tubes 5 mm dia x 65 mm length 10 mm dia x 70 mm length
DAM5M Drosophila Activity Monitor
?32 tubes, 5 mm diameter
?4 independent IR beams per tube
?Reports Counts, Moves, Dwell, Position, Rest
?Assays position preference vs food, odor, light, orientation, etc.
?Built-in clips hold tubes securely
?Available models
DAM2 Drosophila Activity Monitor
?32 tubes, 5 or 7mm diameter
?Dual consolidated IR beams per tube
?Integrated on/off ambient light sensor
?Available models
The DAM2 Drosophila Activity Monitor measures the locomotor activity of 32 individual flies, each in a separate tube. As a fly walks back and forth within its tube, it interrupts an infrared beam that crosses the tube at its midpoint, and this interruption, detected by the onboard electronics, is added to the tube’s activity count as a measure of fly activity. In a typical experiment, an agar/sucrose food mixture is placed into one end of each tube, followed by the fly, and followed by a cotton plug in the open end. The 32 tubes are inserted into holes in the monitor case, and centered. Over the course of the experiment, which may last for days or weeks, the counting circuits continuously monitor the activity in all 32 tubes, and at periodic intervals, upload their count totals to the host computer for storage and later analysis. This daily record provides a good measure of both the intensity of locomotor activity, and the relative periods of rest. The DAM2 is fabricated from a transparent polycarbonate plastic, allowing light penetration to all points along the tube. It is compact, light in weight, and when used within an incubator, allows plentiful air circulation around the tubes. The through-hole design accommodates tubes of arbitrary length. An on/off visible light sensor is built into each unit to provide a simple record of the ambient light state over time. This record accompanies the activity count data as it is acquired and stored, and is useful in analyzing circadian rhythms and adaptations of the flies to external light stimuli. An optional gas distribution manifold is available to facilitate the controlled exchange of air within the tubes, as would be needed for hypoxia studies.
Features
?32 independent activity channels per monitor.
?Up to 120 monitors per system provide over 3000 simultaneous monitoring channels.
?Compact design allows efficient use of incubator shelf space.
?Standard 5mm tube size (model DAM2) for studies of drosophila melanogaster.
?7mm tube size (model DAM2-7) accommodates d. virilis and larger species.
?Ambient light sensor provides on/off record of entrainment cycles and stimulation pulses, as well as inadvertent light exposure.
?Consistent operation in ambient light levels from bright laboratory to total darkness.
?Optional gas distribution manifold allows controlled air exchange within the tubes.
?Available tubes (diameter x length):
PGT5x65 = 5 x 65 mm Pyrex Glass
PGT7x65 = 7 x 65 mm Pyrex Glass
PPT5x65 = 5 x 65 mm Polycarbonate Plastic Plastic caps are available to seal the food in place of the traditional hot wax dip.
Specifications
?Dimensions: 127 x 48 x 87 mm LWH (5.0 x 1.9 x 3.4”)
?Mass: 170g
?Tube diameter: DAM 2 = 5 mm DAM 2-7 = 7 mm
?Ambient Light Sensor threshold: 10 lux nominal, phoic response curve
?Interconnect: 4 wire, 6 position, RJ-11 modular telephone line jack to DAMSystem network for DC power input and data transmission
?Case material: Clear polycarbonate plastic
