Cy?3 AffiniPure Donkey Anti-Rat IgG (H+L)
(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Shp Sr Prot)
Target: Rat
Host: Donkey
Antibody Format: Whole IgG
Specificity: IgG (H+L)
Minimal Cross Reactivity: Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, Sheep Serum Proteins
Conjugate: Cyanine Cy?3
Product Category: Whole IgG Affinity-Purified Antibodies
Clonality: Polyclonal
RRID: AB_2340667
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit and sheep serum proteins, but it may cross-react with immunoglobulins from other species.
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Physical State:Freeze-dried solid
Storage and Rehydration:Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration:Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.Expiration date:one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.Buffer:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)Preservative:0.05% Sodium AzideSuggested Working Concentration or Dilution Range:
1:100 - 1:800 for most applications
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
