SP Sepharose Fast Flow cation exchange chromatography resin
SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification.
SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification.
Well established strong cation exchanger.
Based on Sepharose Fast Flow ion exchange resin.
High chemical stability enables proven CIP and sanitization protocols.
Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Streamlined scale-up
SP Sepharose Fast Flow uses the Sepharose Fast Flow ion exchange resin, which is well established in industrial downstream processing. The highly crosslinked agarose resin enables high flow rates and high productivity.
| Parameter | SP Sepharose Fast Flow, 300 mL |
|---|---|
| BioProcess resin | Yes |
| Ligand | Sulphopropyl |
| Particle size, d50V1 | ~90 μm |
| Ion Exchanger Type | Strong cation exchanger |
| Matrix | cross-linked agarose, 6%, spherical |
| Particle Size | 45 μm-165 μm |
| Ionic Capacity | 0.18-0.25 mmol H+/ml medium |
| pH stability, operational2 | 4–13 |
| pH stability, CIP3 | 3–14 |
| pH ligand fully charged4 | Entire pH range |
| Pressure/Flow Specification | 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm |
| Exclusion limit (Mr) (globular proteins) | ~4 × 10? |
| Storage | 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate |
| Chemical stability5 | Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol |
| Pack size | 300 mL |
